EMMPRIN expression of dysplastic and tumor cell lines. A, EMMPRIN expression was evaluated by western blotting analysis of 10 μg of DOK and SCC-9 cell lysates (β-actin was used as loading control). Representative blot is shown. EMMPRIN transcripts in DOK and SCC-9 cells were quantified using quantitative RT-PCR. Columns represent average values from at least three independent experiments carried out in triplicate; bars, SD. * denotes significant difference with p < 0.05. B, Immunofluorescence staining for EMMPRIN in DOK and SCC-9 cells. Cells were counterstained with DAPI (×400). EMMPRIN staining was stronger in the SCC-9 tumor cells compared to the Dysplastic DOK cells.