EMMPRIN and uPA expression in human oral dysplastic and squamous carcinoma lesions. A- EMMPRIN and uPA mRNA expression in oral dysplastic and tumor tissues. RNA was extracted from formalin-fixed paraffin-embedded tissue sections of 6 normal oral mucosa (Normal), 4 intra epithelial neoplasia (Dysplastic), 8 micro-invasive OSCC and 8 invasive OSCC, and were analysed for EMMPRIN and uPA expression using qRT-PCR assays. * denotes significant difference with p < 0.05. B- Immunohistochemical staining of EMMPRIN and uPA in sections of human oral dysplastic, micro-invasive and invasive squamous carcinoma lesions. Tissue sections from patients with oral dysplastic lesions (OIN), micro-invasive lesions (Micro-inv) and invasive oral squamous carcinoma lesions (INV) lesion sections were subjected to single-labeled immunohistochemistry using mouse anti-human EMMPRIN mAb or goat anti-human uPA Ab and counterstained with HES. EMMPRIN and uPA were colocalized, their staining was heterogeneous with particularly high staining at some regions. Staining was more intense in the tumor sections.