Table 1 Primers for detection of HOXA10 mRNA and miR-135a by RT-PCR
From: miRNA-135a promotes breast cancer cell migration and invasion by targeting HOXA10
Primers | Sequence |
|---|---|
miR-135 RF | cgcgtctatggctttttattccta |
anchor RT primer | cgactcgatccagtctcagggtccgaggtattcgatcgagtcgcactttttttttttt |
Universal rev primer | ccagtctcagggtccgaggtattc |
U6F | ctcgcttcggcagcaca |
U6T | aacgcttcacgaatttgcgt |
HOXA10 RT F | ctggtcccctccctctgtc |
HOXA10 RT T | acaacaaataaaccagcaccaag |
β-actin F | ccttcctgggcatggagtcct |
β-actin T | aatctcatcttgttttctgcg |