Figure 3

Peritumoral vascular invasion and NHERF1 expression in grade 2 invasive breast carcinoma. (A) Representative images of peritumoral vascular invasion by H&E and NHERF1 protein expression by immunoistochemistry: (I) a tumor with the absence of peritumoral vascular invasion and (II) with the overexpression of membranous NHERF1, in addition to cytoplasmic localization (arrow). (III) A case showing peritumoral vascular invasion (arrowhead) and (IV) negative expression of membranous NHERF1. Original magnification × 100, inset × 200. (B) Assessment of the peritumoral vascular invasion in a breast tumor section stained with CD31 and NHERF1 antibodies and detected with Alexa Fluor 568 (red) and Alexa Fluor 488 (green) secondary antibodies, respectively. Immunofluorescence analysyis shows a tumor cell cluster within the endothelial-lined vascular space (arrowheads), with strong cytoplasmic NHERF1 expression similarly to the invasive cellular component at right zone of the image. Scale bar = 10 μm.