Figure 6

Quantitation of the organ tropism of the metastatic cells and the metastatic burden within the mouse organs. A. Comparison of the percentage of total organs that contained metastatic cells (Overall Metastatic Spread) for samples 5-9 as assessed by morphological analysis of H+E stained sections. The total number of mouse organs analyzed is indicated by n above each bar. B. Comparison of the percent of lungs, kidneys, brains and livers that contained metastatic cells (Organ Tropism) for samples 5-9 as assessed by morphological analysis of H+E stained sections. C-E. Graphical representation of the metastatic burden calculated for the total number of lungs, kidneys, brains and livers that were analyzed from mice injected with sample 5-9. The number of mouse organs analyzed is indicated by n in each graph. Each bar represents the calculated metastatic burden for one organ. The metastatic burden in each organ was calculated by dividing the pixels present in the metastatic lesion/s (x pixels) by the total pixels comprising the field of view (y pixels) then multiplying by 100 [(x pixels/y pixels)*100], resulting in a percent value. The percent metastatic burden was calculated from the means from 5 fields of view (100× magnification) per organ analyzed. Values are reported as mean +/- SD.