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Figure 4 | BMC Cancer

Figure 4

From: "A novel in vivo model for the study of human breast cancer metastasis using primary breast tumor-initiating cells from patient biopsies"

Figure 4

Detection of micrometastasis of human cancer cells in mouse tissues. A. PCR for a centromeric region in human chromosome 17 was used to detect human cells in mouse organs isolated from mice injected with tumorspheres into the mammary fat pad 3 months post-injection. Human DNA was detected in the lungs, kidneys, brain, bone marrow, and liver. DNA isolated from MCF-7 cells and mouse tail was used as a positive and negative control, respectively. B. PCR for DNA isolated from organs collected from a mouse injected with Matrigel alone was also used as a negative control. C-F. Micrographs representing micrometastasis in the lung, liver, brain and kidney, respectively, collected from mice previously injected with tumorspheres. 100× magnification in all panels. Arrows indicate micrometastases surrounded by normal mouse tissue.

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