Steady-state levels of CXCL12 transcripts in healthy and malignant ovarian tissues. (A) CXCL12 mRNA was detected by conventional PCR and was of the expected size (417 bp) in healthy ovaries (faint signal), benign and invasive ovarian tumors (strong signal) and in CD326+ epithelial cell-enriched malignant ascites samples. By contrast, CXCL12 transcripts were absent from CD326- non epithelial cells. The results presented are from one experiment representative of three carried out. The white vertical line separates lanes not run on the same gel. (B) CXCL12 mRNA levels were quantified by real-time PCR and are expressed as CXCL12/β-actin ratios. The diagram shows the distribution of values and means for EOC samples identified as CXCL12high and CXCL12low/moderate. Each symbol represents an individual sample run in duplicate. The P value presented is that for a two-tailed Student's t test.