Figure 6

Effects of 7 μM PE5 or 5 μM onconase on the expression of Bcl-2, Bax, JNK and XIAP and on the phosphorylation of p46 JNK in NCI/ADR-RES after treatment for 72 h. Protein levels were determined by Western blot analysis (see Methods section). A) Western blot of a representative experiment. B) Densitometric analysis of the GAPDH-normalized immunoblots of each treatment respective to that of untreated cells. JNK and p-JNK refers to the unphosphorylated and phosphorylated p46 JNK isoforms, respectively. White bars indicate untreated cells (control) and black bars and striped bars indicate PE5- and onconase-treated cells, respectively.