Different effects of GSI-treatment on the cell cycle of NSCs or GSCs. (A, B) Comparisons of cell cycle between NSCs (A) and GSCs (B) in the presence of GSI or DMSO at 24 h, 48 h, 72 h using flow cytometry. Data represent as mean ± SD from three independent experiments. (n = 3, 24 h, P = 0.006, 48 h, P = 0.013). *, P < 0.05, **, P < 0.01.