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Figure 5 | BMC Cancer

Figure 5

From: Notch signaling contributes to the maintenance of both normal neural stem cells and patient-derived glioma stem cells

Figure 5

Augmented neurite outgrowth and enhanced differentiation of patient-derived tumor spheres on the blockade of Notch signaling. (A) Photomicrographs of differentiated tumor spheres at 72 h after plated in differentiation conditional medium supplemented with GSI or DMSO. (B, C) Comparison of neurites number (n = 3, P < 0.001) (B) and length (n = 3, P = 0.041) (C) between tumor spheres in the presence of GSI and DMSO. (D, E) Immunofluorescence. Differentiated tumor spheres were stained with anti-GFAP, or anti-MAP2 antibodies after cultured on cover slips in differentiation conditional medium for 7 days. Stained samples were examined under a fluorescence microscope. (F, G) Quantification and comparison of the percentages of neurons (MAP2+) (n = 3, P < 0.001) (F) or astrocytes (GFAP+) (n = 3, P < 0.001) (G) in the total cell number revealed by Hoechst counterstaining, between GSI-treated and control GSCs. Scale bar, 100 μm for A, and 50 μm for D and E. *, P < 0.05, **, P < 0.01.

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