Patient glioma-derived stem cells have the ability to form neurosphere-like colonies and gave rise to the downstream neural cell types of NSCs. (A) Photomicrographs of typical primary tumor spheres from one glioma tissue at 72 h after plating. (B) Undifferentiated primary tumor spheres expressed high levels of Nestin (red), a marker of NSCs. (C, D) The tumor spheres-derived from human glioma were cultured in differentiation conditional medium for 7 days, and differentiated into neural cells expressing specific molecular markers of GFAP (C, red) and MAP2 (D, green). Scale bar, 100 μm in A, and 50 μm in BCD.