Table 1 Primers used for DNA methylation analysis.
From: Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells
Gene | Segment | Primer sequence (5'-3') | Annealing | Product |
|---|---|---|---|---|
|  |  |  | temp.(°C) | size (bp) |
| Â | 1F | GAAGATAATTGTGGTTATTTTTTGGTA | 55 | 574 |
| Â | 1R | CCACCTCCCTAAATAATATTACT | Â | Â |
| Â | 2F | GTTGTTTTTAGTGGTAAAGGATAGA | 55* | 602 |
| Â | 2R | CACACACATCTTTTAACTAAAACT | Â | Â |
PXR | 3F | AGGATTTATTATTTTAAGGAGGGGTT | 55 | 230 |
promoter | 3R | CCTTAAAACAATACCTCTAACCAT | Â | Â |
| Â | 4F | GTAAGATTTGGAGATTTTTTATATTTG | 55 | 318 |
| Â | 4R | CTATCCTTCTCTACTAATAAAAATAC | Â | Â |
| Â | COBRA | GTAGGGAGAATATAATGAGAATAA | 55* | 209 |
| Â | Â | ACTAAAATAAAAACAATACTTCCTCTTCAC | Â | Â |
PXR | F | ATTTTTTTATAGGAGGGTTATGAAA | 55 | 292 |
exon 3 | R | TACACACRAACACCAACTCACATAT | Â | Â |
CYP3A4 | F | GAGTTATGGTGGGTTTTATTTAG | 55 | 438 |
5'UTR | R | TCTACATTTCCATCTAAAATACC | Â | Â |
VDR | 1F | ATAATTTTAGGTTTTAGGAGGTAG | 60 | 400 |
promoter | 1R | CCTAAACTAACCAAACCAAAACTT | Â | Â |
| Â | 2F | GGGTTGTTTTTGTTTGTTAAAAGG | 60 | 373 |
| Â | 2R | CTTATTACCCAAATACTAAACACT | Â | Â |
PRMT1 | F | AGGAGAAAGGGGGGGTTTTGGT | 55* | 273 |
promoter | R | AACCCTTAAAAACTAAAAAACC | Â | Â |