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Figure 2 | BMC Cancer

Figure 2

From: A p53-independent role for the MDM2 antagonist Nutlin-3 in DNA damage response initiation

Figure 2

Nutlin-3 induces stabilisation and phosphorylation of p53, and activates key p53 target proteins. (A) HCT116p53+/+ cells were untreated (treated with DMSO only) (unt) or treated with 100 μM Etoposide (Eto), or 10 μM Nutlin-3 (Nut) for the times indicated before immunoblotting was used to analyze p53 stabilisation. Actin levels were used to assess equal loading. (B) HCT116p53+/+ cells were treated with 100 μM Etoposide (Eto) or 10 μM Nutlin-3 (Nut) for the times indicated before immunoblotting was used to analyse MDM2 and p21 activation. Actin levels were used to assess equal loading. (C) HCT116p53-/- cells were untreated (treated with DMSO only) (unt) or treated 10 μM Nutlin-3 (Nut) for the times indicated before immunoblotting was used to analyze p53 stabilisation. Actin levels were used to assess equal loading.

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