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Figure 6 | BMC Cancer

Figure 6

From: Genome-wide expression patterns associated with oncogenesis and sarcomatous transdifferentation of cholangiocarcinoma

Figure 6

Genes and proteins differentially expressed in sarcomatoid CC and adenomatous CC cells. (A) Real-time RT-PCR analysis of upregulated (left) and downregulated (right) genes selected from the list of top 25 genes differentially expressed in sarcomatoid SCK cells and three adenocarcinomatous CC cell lines. (B) Immunoblot of upregulated (left) and down-regulated (right) proteins in sarcomtoid SCK cells compared to three adenocarcinomatous CC cell lines. *, selected from the top 100 genes differentially expressed in sarcomatoid SCK cells compared to three adenocarcinomatous CC cell lines. Lane 1, Choi-CK cells. Lane 2, Cho-CK cells. Lane 3, JCK cells, Lane 4, SCK cells. (C) Immunoblot of UCHL1. Expression was restored in adenocarcimatous CC cells by treatment of 5 μM of Aza for 4 days, compared to vehicle control (VC). (D) Immunohistochemical staining of down- or upregulated proteins according to tumor dedifferentiation. The representative picture is one of three immunohistochemical staining replicates of different specimens. Well, well-differentiated CC. Mod, moderately differentiated CC. Poor, poorly differentiated CC. Sar, sarcomatoid CC. *, selected from the top 100 genes differentially expressed in sarcomatoid SCK cells compared to three adenocarcinomatous CC cell lines.

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