Figure 4

Immunohistochemical staining of differentially expressed proteins in the CC tissues. (A) Immunohistochemical staining with anti-SPP1, anti-EFNB2, anti-E2F2, anti-IRX3, anti-PTTG1 or anti-PPARγ in NBE, human CC tissues with good differentiation (well), moderate differentiation (mod) or poor differentiation (poor), and HCC tissues. The representative genes were selected from the list of top 25 commonly upregulated genes, according to antibody available for immunohistochemistry. *, selected from only cell-based microarray database. The representative picture is one of three immunohistochemical staining replicates of different specimens. (B) Immunohistochemical staining with anti-KRT-17, anti-UCHL1, anti-IGFBP7 or anti-SPARC in the human CC and HCC tissues. *, selected from only cell-based microarray database. The representative picture is one of three immunohistochemical staining replicates of different specimens