Expression of THO-UAP56 and ALY in breast tumor cell lines. (A) mRNA levels of THOC1, THOC2, THOC3, THOC5, THOC7, UAP56 and ALY were determined by real-time quantitative PCR (RT-PCR) in breast tumor cell lines MCF7, SKBr3 and T47-D and compared to those of the non tumor cell line MCF10-A. Data are representative of two independent experiments, and quantitative PCRs were repeated three times for each one. Errors bars show standard deviation (SD). (B) Immunohistochemical analysis of THOC1 and ALY expression in normal breast tissues and breast carcinomas. Immunohistochemical analysis was performed on formalin-fixed, paraffin-embedded specimens (see Table below). After incubation with antibodies the slides were counterstained with hematoxylin. Bars represent 50 μm. Expression levels of THOC1 and ALY were determined in a semi-quantitative way (negative staining; weak staining and strong nuclear positivity, for more details see Materials and Methods). In the upper panel a representative photograph of normal and tumor tissue is shown. At the bottom negative controls with no primary antibody are included. Inserts show details at higher magnification. The results of the immunohistochemical analysis of 43 samples are summarized in the table below.