Figure 5

Test compounds affect the expression of the pro- and anti-apoptotic proteins, Bad and Mcl-1, respectively. Total Bad and Mcl-1 immunoblots from lysates collected from HeLa cells treated for 16 hour with 50 μM of indicated test compounds (top panel) and fold expression quantified by densitometry (bottom panel). α-tubulin was used as a loading control. Data represent the mean ± SEM of three independent experiments. # indicates statistical significance compared to untreated controls, p ≤ 0.01. C, Control