In vivo Identification and Specificity assessment of mRNA markers of hypoxia in human and mouse tumors

BMC Cancer

Table 1 Summary of Pearson regression coefficients (R) and P values when comparing relative gene expression and relative FAZA retention in small tumor fragments obtained from 4 human FaDu_ddtumors and 4 murine SCCVII tumors.

FaDu_dd	CA9		GLUT1		LDH		GAPDH		LOX		ATF4
	R	P	R	P	R	P	R	P	R	P	R	P
A	0.77	≈10^-4	0.85	3×10^-6	0.72	< 10^-3	0.17	0.48	0.77	< 10^-3	-0.65	0.003
B	0.84	5×10^-6	0.89	< 10^-6	0.63	0.003	0.67	0.001	0.86	10^-6	-0.73	< 10^-3
C	0.48	0.04	0.51	0.02	0.62	0.004	0.33	0.17	0.73	< 10^-3	-0.33	0.17
D	0.68	10^-3	0.75	≈10^-3	0.73	< 10^-3	-0.1	0.69	0.75	< 10^-3	-0.54	0.02
Mean	0.69		0.75		0.67		0.18		0.78		-0.56
Pooled	0.58	< 10^-7	0.74	< 10^-14	0.52	< 10^-6	0.13	0.24	0.76	< 10^-15	-0.45	< 10^-4
SCCVII	CA9		GLUT1		LDH		GAPDH		LOX		ATF4
	R	P	R	P	R	P	R	P	R	P	R	P
A	0.92	< 10^-4	0.94	< 10^-4	0.82	0.002	0.89	< 10^-3	-0.54	0.08	0.70	0.02
B	0.89	< 10^-6	0.76	< 10^-3	0.50	0.03	0.80	< 10^-4	-0.48	0.03	0.49	0.04
C	0.60	0.005	0.60	0.005	0.43	0.06	0.53	0.02	-0.35	0.13	0.08	0.7
D	0.70	< 10^-3	0.47	0.03	-0.10	0.67	0.16	0.51	-0.30	0.20	0.36	0.1
Mean	0.78		0.69		0.41		0.59		-0.42		0.41
Pooled	0.65	< 10^-9	0.64	< 10^-8	0.24	0.04	0.56	< 10^-6	-0.38	0.001	0.39	< 10^-3

Analysis was performed on a per-tumor basis (A, B, C and D). Similar data analysis was also performed after data were pooled, i.e., for each gene the relative gene expression for all fragments for a given tumor type was plotted as function of relative FAZA content followed by regression analysis.

ISSN: 1471-2407