Figure 5

Inhibitory effects of baicalein on the motility and invasiveness of A431 cells. A431 cells were treated with 20 μM bacailein, and cell invasiveness was detected using Boyden Chamber with Matrigel coating. A-(a), Invasiveness of control cells (treated with 0.1% DMSO). A-(b), Invasiveness of cells treated with 20 μM baicalein. Scale bar, 10 μm. A-(c), Numbers of cells invading through the filters of chamber (10 fields). To test for dose-dependence, A431 cells were treated with baicalein at 20 or 40 μM for 48 h, and for the time-course experiment, A431 cells were treated with 20 μM baicalein for 24, 48, and 72 h. The cells were then subjected to analyses for motility and invasion abilities as described in Methods. B. Motility of A431 cells at various concentrations of baicalein. C. Invasiveness of A431 cells at various concentrations of baicalein. D. Motility of A431 cells at various time points. E. Invasion of A431 cells at various time points. The data are represented as the mean ± SD from three independent experiments. Results were statistically analyzed using one-way ANOVA with post hoc Dunnett's test (* p < 0.05). The error bars represent SDs. IR, invasion rate. MR, motility rate.