Figure 4
Inhibitory effects of baicalein on A431 cell migration. A431 cells (2 × 106) were seeded in 10-mm plates and incubated at 37°C for 48 h. Confluent monolayers of A431 cells were then wounded using a plastic tip. The cells were treated with 20 μM baicalein, then photographed after 48 h. The cells migrating cross the boundaries lines in the center of the wells were counted, and Ezrin expression was examined in the cell samples. (a) A431 cells treated with 0.1% DMSO. (b) A431 cells treated with 20 μM baicalein. (c) A431 cells transfected with Ezrin si-RNA. (d) Numbers of cells that moved cross the lines (10 fields). (e) Ezrin expression in A431 cells with 0.1% DMSO, 20 μM baicalein or Ezrin si-RNA, respectively. The data are represented as the mean ± SD from three independent experiments. Results were statistically analyzed using one-way analysis of variance (ANOVA) with a post hoc Dunnett's test (* p < 0.05). The error bars represent SDs.