NSE knockdown reduces the migration of glioma cells. (A) U251 and A172 were transfected with control siRNA or NSE siRNA. NSE protein level was assessed by Western blot. GAPDH was the loading control. (B) NSE knockdown in A172 and U251 slightly decreased the proliferation of the cells ( 2-17% ). (C) ECIS experiment showed NSE knockdown reduced the migration of A172. (D) The ratio of impedance before the wounding and after the wounding until the end of the experiment in both the control and NSE siRNA treatment groups. Error bars represent the SEM. Experiments were performed three times. *P< 0.05. Ctrl, control siRNA.