Figure 3

5-dAzaC up-regulates HSD17B1 transcript levels in HT29 (A) and SW707 (B) colorectal cancer cells. HT29 and SW707 cells were cultured in phenol red-free DMEM for 6, 12, 24, 36 and 48 h either in the absence or in the presence of 5-dAzaC at a concentration of 1.00 μM. After incubation the cells were used for total RNA isolation and reverse transcription. The HSD17B1 cDNA levels were determined by RQ-PCR relative quantification analysis. RQ-PCR results were standardized by the geometric mean of PBGD and hMRPL19 cDNA levels. HSD17B1 cDNA levels are expressed as a multiplicity of the respective controls. Each sample was determined in triplicate and results are presented as the mean ± SE from three experiments *P < 0.05.