Skip to main content
Figure 4 | BMC Cancer

Figure 4

From: Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation

Figure 4

17-AAG, EC154, and LBH589 suppress HIF-dependent reporter gene expression and VEGF secretion. (a) CCRCC cells were transiently transfected with either a constitutive or VEGF HRE-driven luciferase reporter construct, treated for 16 h with inhibitors, and luciferase enzyme activity determined in whole cell lysates. All drug treatments significantly reduced HIF-dependent reporter gene expression (*) in both cell types with the exception of EC154 in 786-O, as determined by ANOVA and Student's t-test (p < 0.05). (b) CCRCC cells were pre-treated for 4 h with inhibitors in reduced serum DMEM (3% FBS), and incubated for an additional 16 h with freshly prepared treatments in reduced serum medium. Conditioned medium was collected and VEGF levels were analyzed by ELISA. Values were normalized to total protein in conditioned medium and presented relative to controls, with standard deviation. All drug treatments significantly reduced VEGF secretion (*) in both cell types, as determined by ANOVA and Student's t-test (p < 0.05).

Back to article page