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Figure 1 | BMC Cancer

Figure 1

From: Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation

Figure 1

HIF-1α and HIF-2α expression is differentially modulated by Hsp90 inhibition in CCRCC cells. UMRC2 and 786-O cells were treated for 20 h with the Hsp90 inhibitors 17-AAG (1 μM), EC154 (100 nM), the HDAC inhibitor LBH589 (100 nM), or DMSO vehicle control. Nuclear protein was analyzed for HIF-1α and HIF-2α and cytosolic protein was analyzed for RACK1 by SDS-PAGE and Western blot. Topoisomerase II and GAPDH were used as nuclear and cytosolic loading controls, respectively. Bands corresponding to HIF-1α and HIF-2α Western blots were quantified, normalized to TOPOII, and are expressed as a percentage of control samples.

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BMC Cancer

ISSN: 1471-2407

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