Figure 1From: A novel tumor suppressor gene ECRG4 interacts directly with TMPRSS11A (ECRG1) to inhibit cancer cell growth in esophageal carcinoma The interaction between ECRG4 and ECRG1 was examined by binding affinity assay in vitro. FLAG-ECRG1 from EC9706/pcDNA3.1-FLAG-ECRG1 cells was bound to recombinant His-ECRG4 protein which had been pre-coated on the plate. The absorbance values of the wells (column 6) were significantly higher than those of controls (P < 0.001). And no detectable binding of Miz-1, an interaction partner of ECRG1, to His-ECRG4 was observed. *, P < 0.001, compared with EC9706/pcDNA3.1-FLAG cells.Back to article page