Figure 5

Western blot quantification and protein yield calculations. Western blot quantification was performed using Scion Image software. Depicted are the signal intensities of anti-PSA (a) and anti-GOLPH2 (b) antibodies, normalized to beta-Actin. The quantification of the western blots revealed for most of the samples no differences between the signal intensities from cryopreserved, HOPE-fixed or formalin-fixed samples. FFPE showed for some cases even the strongest signal which might be dependent on the epitope detected by the specific antibody or due to a very weak beta-Actin staining for those FFPE samples. For protein yield calculations (c) the area of the sections was measured using AxioVision Rel. 4.8 and the yield was calculated for each sample. The resulting protein yields were similar or even higher for HOPE and FFPE tissues compared to cryopreserved samples.