Western blot analysis of FFPE, HOPE, and fresh-frozen samples using PSA (34 kDa), GOLPH2 (45-50 kDa), and beta-actin (42 kDa) antibodies. Eighteen μg of protein was separated by SDS-PAGE and western blot analysis was performed using indicated antibodies. Strong bands could be detected at 34 kDa with the anti-PSA antibody (a) and at 45-50 kDa with the anti-GOLPH2 antibody (b). The housekeeping gene beta-actin also showed clear bands at 42 kDa. Signal intensities of HOPE-fixed tissue specimens were at least as intense as those of cryopreserved and FFPE samples.