Confluence-dependent upregulation of transcription factors in HCT-116 and HCT-15 cells. Culture conditions were the same as for Figure 1. Protein expression was analyzed by Western blotting as described in Materials and Methods. Data shown are representative of three independent experiments. All transcription factors exhibited altered protein expression levels in a confluence-dependent manner except for HNF-1α, which was unchanged. *N/D, Cdx2 was not detected in HCT-116.