Table 2 Characteristics of the PCR-RFLP methods used
From: Pharmacogenetic profiling and cetuximab outcome in patients with advanced colorectal cancer
| Gene | SNP |
Restriction Enzyme | Primers |
|---|---|---|---|
| EGFR |
497R > K rs2227983 | BstNI |
5-TGCTGTGACCCACTCTGTCT-3 5-CCAGAAGGTTGCACTTGTCC-3 |
| EGFR |
-216 G > T rs712829 | BseRI |
5-CCACCGCCTCCGGCGGCCGCTGGCCTTG-3 5-CGGCGAGACACGCCCTTACCTTT-3 |
| EGFR |
-191C > A rs712830 | SacII |
5-CCACCGCCTCCGGCGGCCGCTGGCCTTG-3 5-CGGCGAGACACGCCCTTACCTTT-3 |
| EGF |
61A > G rs4444903 | AluI |
5-TGTCACTAAAGGAAAGGAGGT-3 5-TTCACAGAGTTTAACAGCCC-3 |
| CCND1 |
870A > G rs603965 | ScrFI |
5-GTGAAGTTCATTTCCAATCCGC-3 5-GGGACATCACCCTCACTTAC-3 |
| FCGR2A |
131R > H rs1801274 | BstUI |
5-GGAAAATCCCAGAAATTCTCGC-3 5-CAACAGCCTGACTACCTATTACGCGGG-3 |
| FCGR3A |
158F > V rs396991 | NlaIII |
First PCR: 5-ATATTTACAGAATGGCACAGG-3 5-GACTTGGTACCCAGGTTGAA-3 |
|
Second PCR: 5-ATCAGATTCGATCCTACTTCTGCAGGGGGCAT-3 5-ACGTGCTGAGCTTGAGTGATGGTGATGTTCAC-3 |