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Table 2 Characteristics of the PCR-RFLP methods used

From: Pharmacogenetic profiling and cetuximab outcome in patients with advanced colorectal cancer

Gene SNP Restriction
Enzyme
Primers
EGFR 497R > K
rs2227983
BstNI 5-TGCTGTGACCCACTCTGTCT-3
5-CCAGAAGGTTGCACTTGTCC-3
EGFR -216 G > T
rs712829
BseRI 5-CCACCGCCTCCGGCGGCCGCTGGCCTTG-3
5-CGGCGAGACACGCCCTTACCTTT-3
EGFR -191C > A
rs712830
SacII 5-CCACCGCCTCCGGCGGCCGCTGGCCTTG-3
5-CGGCGAGACACGCCCTTACCTTT-3
EGF 61A > G
rs4444903
AluI 5-TGTCACTAAAGGAAAGGAGGT-3
5-TTCACAGAGTTTAACAGCCC-3
CCND1 870A > G
rs603965
ScrFI 5-GTGAAGTTCATTTCCAATCCGC-3
5-GGGACATCACCCTCACTTAC-3
FCGR2A 131R > H
rs1801274
BstUI 5-GGAAAATCCCAGAAATTCTCGC-3
5-CAACAGCCTGACTACCTATTACGCGGG-3
FCGR3A 158F > V
rs396991
NlaIII First PCR:
5-ATATTTACAGAATGGCACAGG-3
5-GACTTGGTACCCAGGTTGAA-3
    Second PCR:
5-ATCAGATTCGATCCTACTTCTGCAGGGGGCAT-3
5-ACGTGCTGAGCTTGAGTGATGGTGATGTTCAC-3