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Table 1 RT-PCR analysis of marker gene expression in L50 tumour samples compared to HGT-1 tumours

From: Adaptation to statins restricts human tumour growth in Nude mice

Lipids synthesis HMG-CoA reductase 0.7 ± 0.1*
  SREBP-1 0.6 ± 0.2*
  SREBP-2 n.c.
  LDL receptor 0.3 ± 0.06***
  FAS 0.6 ± 0.1**
Cell proliferation Cyclin D1 0.7 ± 0.2***
  Cyclin B1 0.66 ± 0.3*
  P21 n.c.
  EGFR n.c.
Apoptosis Caspase 2 n.c.
  Caspase 3 1.5 ± 0.4**
  Caspase 6 1.3 ± 0.2**
  Caspase 7 3.9 ± 0.6***
  Caspase 9 n.c.
  Bax 1.3 ± 0.3*
  Bcl2 1.6 ± 0.4**
  Mcl-1 n.c.
  1. Real Time RT-PCR was performed with mRNA samples extracted from 10 tumours from independent mice. The fold variation was determined with the ΔΔCt method [16]. HGT-1 mRNA levels were set to 1. The mRNA levels in L50 tumours were expressed relative to those in HGT-1 tumours. * Statistical difference (p < 0.05); ** Statistical difference (p < 0.01); *** Statistical difference (p < 0.001) (Student's t test) n.c. no change