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Table 1 RT-PCR analysis of marker gene expression in L50 tumour samples compared to HGT-1 tumours

From: Adaptation to statins restricts human tumour growth in Nude mice

Lipids synthesis

HMG-CoA reductase

0.7 ± 0.1*

 

SREBP-1

0.6 ± 0.2*

 

SREBP-2

n.c.

 

LDL receptor

0.3 ± 0.06***

 

FAS

0.6 ± 0.1**

Cell proliferation

Cyclin D1

0.7 ± 0.2***

 

Cyclin B1

0.66 ± 0.3*

 

P21

n.c.

 

EGFR

n.c.

Apoptosis

Caspase 2

n.c.

 

Caspase 3

1.5 ± 0.4**

 

Caspase 6

1.3 ± 0.2**

 

Caspase 7

3.9 ± 0.6***

 

Caspase 9

n.c.

 

Bax

1.3 ± 0.3*

 

Bcl2

1.6 ± 0.4**

 

Mcl-1

n.c.

  1. Real Time RT-PCR was performed with mRNA samples extracted from 10 tumours from independent mice. The fold variation was determined with the ΔΔCt method [16]. HGT-1 mRNA levels were set to 1. The mRNA levels in L50 tumours were expressed relative to those in HGT-1 tumours. * Statistical difference (p < 0.05); ** Statistical difference (p < 0.01); *** Statistical difference (p < 0.001) (Student's t test) n.c. no change