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Figure 1 | BMC Cancer

Figure 1

From: Characterization of a novel PTEN mutation in MDA-MB-453 breast carcinoma cell line

Figure 1

Aberrant PTEN protein species in MDA-MB-453. (A) Western blotting analysis of PTEN in human breast cancer cell lines. The faster mobility species in MDA-MB-453 is highlighted with an arrow. Actin was used as a loading control. (B) Sequencing analysis of exon8 of PTEN using genomic DNA and cDNA derived from MDA-MB-453 showing the G to A substitution at codon 307 altering the glutamate (E) residue to a lysine (K). (C) Schematic representation of PTEN depicting the catalytic domain (CAT) and the C2 loop with the ubiquitination (Ub) site at K289 and the E307K mutation shown. (D) Both wild-type (WT) and E307K mutant were transfected in a PTEN-null cell line, PC3. Note the faster migrating E307K mutant. All membranes were probed with the monoclonal anti-PTEN antibody (A2B1).

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