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Table 3 Early apoptosis in HeLa, SiHa, or HaCaT cells after in vitro exposure to pentoxifylline or cisplatin either alone or in combination.

From: Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence

HeLa

GROUP

ANNEXIN V

ELISA

 

(% mean ± SD)

(% mean ± SD)

CONTROL

3.7 ± 1.0

10.0 ± 1.5

PTX 8 mM

30.5 ± 1.1 *

30.3 ± 2.3 *

CIS 4 μM

10.9 ± 1.4 *

10.0 ± 2.3 *

PTX + CIS

25.2 ± 1.3 *

20.1 ± 2.5 *

SiHa

GROUP

ANNEXIN V

ELISA

 

(% mean ± SD)

(% mean ± SD)

CONTROL

3.7 ± 1.0

10.0 ± 1.5

PTX 8 mM

28.4 ± 2.1 *

20.5 ± 1.8 *

CIS 4 μM

17.0 ± 0.2 *

17.5 ± 2.7 *

PTX + CIS

35.2 ± 1.0 *

30.5 ± 1.5 *

HaCaT

GROUP

ANNEXIN V

ELISA

 

(% mean ± SD)

(% mean ± SD)

CONTROL

3.8 ± 0.2

10.0 ± 1.5

PTX 8 mM

6.0 ± 0.7

10.3 ± 1.8

CIS 4 μM

16.3 ± 0.7 *

20.1 ± 2.3 *

PTX + CIS

13.5 ± 1.0 *

15.4 ± 1.5 *

  1. Cell cultures were treated with PTX or CIS or their combination, 24 hours later the cells were harvested and early apoptosis was determined by flow cytometry (annexin V-FITC) or ELISA kit (DNA-histone nucleosome). The results represent the mean ± SD of three independent experiments carried out in triplicate. Statistical analysis Student t test. (*) P <0.001 vs untreated control cells.
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BMC Cancer

ISSN: 1471-2407

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