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Table 3 Early apoptosis in HeLa, SiHa, or HaCaT cells after in vitro exposure to pentoxifylline or cisplatin either alone or in combination.

From: Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence

HeLa
GROUP ANNEXIN V ELISA
  (% mean ± SD) (% mean ± SD)
CONTROL 3.7 ± 1.0 10.0 ± 1.5
PTX 8 mM 30.5 ± 1.1 * 30.3 ± 2.3 *
CIS 4 μM 10.9 ± 1.4 * 10.0 ± 2.3 *
PTX + CIS 25.2 ± 1.3 * 20.1 ± 2.5 *
SiHa
GROUP ANNEXIN V ELISA
  (% mean ± SD) (% mean ± SD)
CONTROL 3.7 ± 1.0 10.0 ± 1.5
PTX 8 mM 28.4 ± 2.1 * 20.5 ± 1.8 *
CIS 4 μM 17.0 ± 0.2 * 17.5 ± 2.7 *
PTX + CIS 35.2 ± 1.0 * 30.5 ± 1.5 *
HaCaT
GROUP ANNEXIN V ELISA
  (% mean ± SD) (% mean ± SD)
CONTROL 3.8 ± 0.2 10.0 ± 1.5
PTX 8 mM 6.0 ± 0.7 10.3 ± 1.8
CIS 4 μM 16.3 ± 0.7 * 20.1 ± 2.3 *
PTX + CIS 13.5 ± 1.0 * 15.4 ± 1.5 *
  1. Cell cultures were treated with PTX or CIS or their combination, 24 hours later the cells were harvested and early apoptosis was determined by flow cytometry (annexin V-FITC) or ELISA kit (DNA-histone nucleosome). The results represent the mean ± SD of three independent experiments carried out in triplicate. Statistical analysis Student t test. (*) P <0.001 vs untreated control cells.