Phosphorylation of the IκBα [pS32] and IκBα (Total) by ELISA kit of HeLa and SiHa cells after in vitro treatment with pentoxifylline or cisplatin either alone or in combination. 24 hours later the cells were harvested and the phosphorylation of the IκBα [pS32] and IκBα (Total) was determined by commercial ELISA kit (Invitrogen). The results represent the mean ± SD of three independent experiments carried out in triplicate. Statistical analysis Student's t test. (*) P <0.001 vs CTL. (•) P <0.001 vs TNF-α. (♦) P <0.001 vs CIS.