Figure 6

Knockdown of PDGFRs inhibited invasion in recurrent cell lines. Matrigel coated chambers were used to assess cell invasion after PDGFR siRNA. Cells were transfected with siRNA and 24 h later, cells were lifted, resuspended in serum free media and added to the upper chamber of the matrigel insert; full serum media was added to the bottom well. After 24 h, cells passing through the matrigel coated membrane were fixed and stained with toluidine blue. Representative images for each condition are shown (a). Scale bar = 50 μm. The average number of invading cells per field of view was calculated and related back to respective controls (b). Results represent the average of 3 replicates. * = p < 0.05.