Figure 5

PDGFR knockdown inhibited cell migration in IGF-IR-independent recurrent cells. PDGFRα and β were knocked down transiently using siRNA alone or in combination 48 h after plating either RJ348 or RJ423 cells (depicted in a and b respectively). Four hours later a pipette tip was used to scratch a wound down the middle of the cultured cells (time 0 h, depicted above). Images were captured at 40 × magnification using an inverted bright field microscope at varying time points after the scratch was made. Representative results are shown; the assay was repeated four times. Scale bars = 1 mm.