Figure 3

Dynamics of GnRH receptor activation following treatment with Triptorelin. Treatment of stably transfected cells with GnRH elicited high levels of ³H- inositol phosphate (IP) production (A-C). Removal of GnRH and LiCl revealed the dynamics of ³H- inositol phosphate turnover in different cell types (D,E). The decrease in levels of ³H- inositol phosphates was slower in SVCT-2 cells. Statistically different values (p < 0.05 ANOVA followed by Dunnett's test) compared to control values were as follows; for A, All values shown for SVCT-2 and HEK293_[SCL60] cells > -10 log [peptide]; for B, all values shown > -10 log [peptide] for all 3 cell lines; for C, all values shown > -10 log [peptide] for both cell lines; for D, all values shown for SVCT-2 and all values up to 180 min for WPE-1-NB26-8; for E, all values from 30 min to 180 min.