Effect of overexpression of Hakai on the levels and localization of FAK, Paxillin and Vinculin. (A) Expression of FAK, Paxillin and Vinculin in parental and in several Hakai-overexpressing MDCK checked clones (described in methods). Lysates prepared from the indicated cells were examined by Western blot analysis to detect the proteins shown. (B) Effect of overexpression of Hakai on the localization of FAK and Paxillin. Parental and Hakai-overexpressing MDCK cells were examined by immunofluorescence with anti-FAK and anti-Paxillin antibodies. Scale bars, 10 μm.