Figure 5

α-TEA inhibits tumor growth without modulating HER2/ neu expression. SCID mice with established MDA-MB-453 tumors (day 15 post-tumor injection) received α-TEA in the diet (~2 mg/day/mouse) or 40 μg trastuzumab by i.p. injection, every 2 to 3 days for a total of 7 injections. On day 30 post-tumor injection, tumors were mechanically dissociated and a single cell suspension was prepared and analyzed for HER2/neu expression by flow cytometry. HER2/neu expression of MDA-MB-453 tumor cells used for transplantation is shown for comparison (pre-injection). Cells were gated on light scatter and non-viable cells were excluded from the analysis using viability stain.