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Figure 2 | BMC Cancer

Figure 2

From: Development and characterization of a novel C-terminal inhibitor of Hsp90 in androgen dependent and independent prostate cancer cells

Figure 2

Selective KU174 mediated client protein degradation in the absence of heat shock protein induction. PC3-MM2 (Figure 2A and 2B) and LNCaP (Figure 2C) were examined for client protein degradation and the induction of heat shock proteins. 17-AAG demonstrates Hsp induction (Figure 2A) in PC3-MM2 while KU174 triggers degradation of Hsc70, HspHsp27 and HSF-1 with induction of GRP94 and HspHsp60 in PC3-MM2 cells. Notably, KU174 induced a dose-dependent decrease in several known proteins (Akt, Her2, nestin and CRCX4) that have been shown to play a role in the etiology of prostate cancer. A similar trend of client protein degradation was seen in LNCaP however this cell line was generally more sensitive to inhibitors (Figure 2C). Rapid client protein degradation within 6 hours was seen in both cell lines (Figure 2D).

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