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Figure 5 | BMC Cancer

Figure 5

From: Genome wide single cell analysis of chemotherapy resistant metastatic cells in a case of gastroesophageal adenocarcinoma

Figure 5

ERBB2 and KRAS gene amplification. Open arrows indicate examples of non-amplified cells, whereas closed arrows point to cells with gene amplification. Images were taken with a Zeiss Axioplan 2 fluorescence microscope objective ×60. (A) Fluorescence in situ hybridization of the ERBB2 gene (red) in the primary gastroesophageal tumor shows amplified signals as compared to the reference centromere 17 (green). (B) FISH analysis of ERBB2 (red) and centromere 17 (green) in an iliac bone metastasis progressing during chemotherapy. (C) FISH analysis of the KRAS gene (red) and centromere 12 (green) in the primary gastroesophageal tumor. (D) FISH analysis of KRAS (red) and centromere 12 (green) in an iliac bone metastasis progressing during chemotherapy. One positive cell is displayed at ×2,5 higher magnification than cells in A-C. Blue color indicates DAPI nuclear counterstaining.

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