Figure 4

Biopsies obtained from the primary oesophageal tumor, liver metastasis and right iliac bone metastasis of the 35-year-old male at the time of diagnosis, and from a corresponding right iliac bone metastasis after three cycles of chemotherapy. (A) Hematoxylin and eosin (HE) staining of the primary oesophageal tumor and corresponding immunohistochemical c-erbB2 staining (brown) of the same biopsy (B-D), where both positive and negative tumor cells are seen (C). (B) A close-up of C showing erbB2 negative tumor cells. (D) A close-up of C showing erbB2 positive tumor cells. (E) HE staining of a bone biopsy sampled at the time of diagnosis. (F) erbB2 staining of the same bone biopsy as E, showing only weakly positive staining (1+) and termed clinically negative. (G) HE staining of a liver metastasis biopsy. (H) erbB2 staining of the same liver metastasis biopsy, showing only weakly positive staining (1+) and termed clinically negative. (I) HE staining of a biopsy from bone metastasis of the right iliac bone sampled after three cycles of chemotherapy. (J) erbB2 immunohistochemical staining of the same biopsy as I, showing bone marrow infiltrated with tumor cells strongly positive (3+) for erbB2.