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Table 2 74 cell lines ordered based on the protein level of Ruc-GFP derived by FACS Data (infectivity index)

From: Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68

Cell line

Ruc-GFP Index

1/Δ Ct

Cell line

Ruc-GFP Index

1/Δ Ct

*MOLT4

242.25

0.05

SNB75

22849.59

0.114

*HL-60

890.01

0.038

OVCAR-3 p42

26919.33

0.102

*CCRF-CEM

890.69

0.095

MDA-MB231 p41

30471.13

#

*K562

1185.59

0.066

OVCAR-8

31066.5

0.142

*SW-620

1223.86

#

ACHN

31639.56

#

*SR

1389.99

0.079

RXF-393

35969.66

0.095

*NCI-H522

1723.7

0.081

EKVX

36117.84

0.111

*OVCAR5

1961.62

#

A498

36486.43

#

*HCT15

2121.68

0.104

Huh 7.5.1

39321.66

0.116

*LOX-IMVI

2591.66

#

NCI-H23

40056.95

#

T47D

2831.5

0.093

PC3 p35

40124.72

#

NCI-H1299

3499.99

#

SF539

46092.96

#

RPMI-8226

3891.89

0.085

NCI-ADR_RES

46569.81

0.138

HOP92

4430.72

#

TK-10

49256.78

0.136

UO-31

5960.36

#

SN12C

50505.78

0.175

A375

6049.59

0.094

MCF-7

55230.93

0.119

HCC2998

6537.28

0.093

SF 295

56037.99

#

786-0

7141.29

0.094

888-MEL

56437.28

0.131

MDA-MB 231

7500.93

#

DU-145

56733.35

0.15

Panc1

7876.6

#

KM12

57112.82

0.144

SK-Mel 5

7917.7

0.089

PC3 p7

57919.09

0.118

SF268

8703.17

#

NCI-H322M

69687.9

0.229

NCI-H460

9247.41

0.097

Siha

72182.24

#

UACC 62

10437.13

0.092

SK-OV-3

73220.28

0.129

U-251

11245.39

#

SK-MEL2

75407.23

0.133

NCI-H226

12182.98

0.108

HT29 p155

84833.55

#

Hs578T

13912.24

#

M14

94724.62

0.171

GI-101A

14791.4

#

*Colo 205

101576.11

0.142

UACC 257

15405.44

0.105

*OVCAR-4

111530.36

0.141

Caki I

15869.9

0.102

*1858-MEL

129262.97

0.164

SK-Mel 28

16737.85

0.097

*397-MEL

134615.99

0.139

MIA PaCa2

17376.47

#

*IGR-OVI I

141059.48

0.147

HOP62

19768.46

0.126

*HT29 p6

159709.89

#

MDA-MB435

20698.92

#

*HCT 116

406258.9

0.141

1936-MEL

21079.94

#

*A549 p120 avg

409912.11

0.25

SNB19

21349.31

#

*OVCAR-3 p7

425559.88

#

BT549

22182.84

0.113

*A549 p4

425962.87

#

  1. To test the correlation between protein and mRNA levels, expression of Ruc-GFP was screened by RT-PCR in 46 cell lines post-infected. RT-PCR values are expressed as 1/ΔCt (Ct GFP-Ct 18S). Cells further used in microarray class comparison and class prediction analysis are indicated with *.