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Table 2 Real-time PCR

From: Effects of EpCAM overexpression on human breast cancer cell lines

  Expression of
  TBP SRFP1 TCF7L2 ITF-2
n = 3 Ct ± SD CV[%] Ct ± SD CV[%] Ct ± SD CV[%] Ct ± SD CV[%]
MDA-MB-231 control 22.11 ± 1.90 4.95 31.42 ± 2.90 9.22 19.19 ± 1.45 7.54 23.47 ± 0.43 1.82
MDA-MB-231 EpCAM 21.81 ± 0.10 0.47 34.37 ± 1.80 5.25 20.75 ± 0.27 1.33 36.26 ± 5.50 15.18
  comparison EpCAM expressing versus control
Regulation Factor    -11.06 -3.64 -1773.84
REST analysis
P(H1)    0.000 0.000 0.000
Result    DOWN DOWN DOWN
  Expression of
  TBP SRFP1 TCF7L2 ITF-2
n = 4 Ct ± SD CV[%] Ct ± SD CV[%] Ct ± SD CV[%] Ct ± SD CV[%]
Hs578T control 23.23 ± 0.72 3.09 25.35 ± 2.07 8.17 21.28 ± 0.86 4.04 22.73 ± 1.81 7.96
Hs578T EpCAM 22.54 ± 0.18 0.81 28.50 ± 3.39 11.91 20.76 ± 0.56 2.71 23.42 ± 0.54 2.30
  comparison EpCAM expressing versus control
Regulation Factor    -35.82 -0.73 -3.55
REST analysis
P(H1)    0.048 0.658 0.128
Result    DOWN not different to control not different to control
  1. Quantification of differential mRNA expression of the Wnt signaling components SFRP1, TCF7L2 and ITF-2 in the stable EpCAM expressing cells in comparison to the empty vector control lines. (SD = standard deviation, CV = coefficient of variation, REST P(H1) = hypothesis test p value, significant if < 0.05).