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Table 2 Real-time PCR

From: Effects of EpCAM overexpression on human breast cancer cell lines

 

Expression of

 

TBP

SRFP1

TCF7L2

ITF-2

n = 3

Ct ± SD

CV[%]

Ct ± SD

CV[%]

Ct ± SD

CV[%]

Ct ± SD

CV[%]

MDA-MB-231 control

22.11 ± 1.90

4.95

31.42 ± 2.90

9.22

19.19 ± 1.45

7.54

23.47 ± 0.43

1.82

MDA-MB-231 EpCAM

21.81 ± 0.10

0.47

34.37 ± 1.80

5.25

20.75 ± 0.27

1.33

36.26 ± 5.50

15.18

 

comparison EpCAM expressing versus control

Regulation Factor

  

-11.06

-3.64

-1773.84

REST analysis

P(H1)

  

0.000

0.000

0.000

Result

  

DOWN

DOWN

DOWN

 

Expression of

 

TBP

SRFP1

TCF7L2

ITF-2

n = 4

Ct ± SD

CV[%]

Ct ± SD

CV[%]

Ct ± SD

CV[%]

Ct ± SD

CV[%]

Hs578T control

23.23 ± 0.72

3.09

25.35 ± 2.07

8.17

21.28 ± 0.86

4.04

22.73 ± 1.81

7.96

Hs578T EpCAM

22.54 ± 0.18

0.81

28.50 ± 3.39

11.91

20.76 ± 0.56

2.71

23.42 ± 0.54

2.30

 

comparison EpCAM expressing versus control

Regulation Factor

  

-35.82

-0.73

-3.55

REST analysis

P(H1)

  

0.048

0.658

0.128

Result

  

DOWN

not different to control

not different to control

  1. Quantification of differential mRNA expression of the Wnt signaling components SFRP1, TCF7L2 and ITF-2 in the stable EpCAM expressing cells in comparison to the empty vector control lines. (SD = standard deviation, CV = coefficient of variation, REST P(H1) = hypothesis test p value, significant if < 0.05).