Effect of Nck2 on human primary melanoma cell migration. Human primary melanoma cell migration was evaluated using wound healing assays on WM278 cells overexpressing GFP (C2) or increasing levels of GFP-Nck2 (N15 < N7 < N14). Pictures from the same area were taken at time 0 and 8 hours after the wound. Magnification: 10X and white bar = 200 μm. Quantification of migration is expressed as percentage of closed wound ± SD. * p < 0.001 vs C2 using Student's t-test.