Figure 3From: Nck2 promotes human melanoma cell proliferation, migration and invasion in vitro and primary melanoma-derived tumor growth in vivo Effect of Nck2 on human primary melanoma cell proliferation. (A) Total cell lysates from stable WM278 human primary melanoma cells overexpressing GFP (C2) or increasing levels of GFP-Nck2 (N15 < N7 < N14) were subjected to western blot analysis using GFP or Nck2 specific antibodies. Cell proliferation of (B) WM278 cells stably overexpressing GFP (C2) or GFP-Nck2 (N15, N7 and N14), as well as (C) parental primary (WM278) and metastatic (WM1617) melanoma cells was determined by quantification of Crystal Violet incorporation each day during 4 days in culture. Results are shown as the ratio of the mean of cell density over day 1 ± SD (n = 4). * p < 0.02, ** p < 0.006 and ***p ≤ 0.0001compared with C2 (B) or parental WM278 cells (C) using Student's t-test. (D) Nck1 and CrkII expression was evaluated using total cell lysates (30 μg protein) prepared from WM278 cells stably overexpressing GFP (C2) or GFP-Nck2 (N15, N7, and N14). β-tubulin was used as loading control.Back to article page