Figure 9
Interferon response is essential for limiting RSV infection in prostate cells. (a) RSV infection of RWPE-1 in the absence (untreated, UT) or in the presence of either control (Con Ab) or IFN neutralizing (IFN Ab) antibody. Infection was measured by plaque assay at 36 h post-infection. Plaque assay values expressed as pfu/ml represent mean ± standard deviation for three independent determinations. Standard deviations are shown by error bars. (b) Plaque assay showing RSV infectivity in Con Ab or IFN Ab treated cells. Culture supernatant from RSV infected RWPE-1 cells (+/- Con Ab or IFN Ab) was added to CV-1 cells at a dilution of 1 × 106. Plaques were observed on methyl-cellulose after crystal-violet staining.