Impaired IFNα- and RSV-mediated STAT-1 activation in LNCaP cells. STAT-1-specific EMSA of nuclear extracts prepared from mock-infected and RSV-infected LNCaP and RWPE-1 cells (a) PC-3 cells (b). The STAT-1/DNA EMSA complex is indicated with arrowheads. (c) STAT-specific EMSA of nuclear extracts prepared from cells treated with (+) or without (-) IFNα for 4 hours. Asterisks represent STAT-1/DNA complex. NS: non specific band.