NF-κB activation in RSV infected prostate cancer cells. NF-κB specific EMSA of nuclear extracts prepared from mock-infected and RSV-infected LNCaP (a) RWPE-1 (b) and PC-3 (c) cells. Duration of infection (in hours) with RSV is indicated. The NF-κB/DNA EMSA complex is indicated with arrowheads. Competition of the EMSA complex with the cold NF-κB duplex oligonucleotide, but not with cold STAT1/2 oligo duplex in panel (a) ensures the specific nature of the EMSA complex.