Oncolytic activity of RSV. (a) RSV infection of RWPE-1, LNCaP and RM1 cells were measured by plaque assay at 36 h post-infection. Plaque assay values expressed as pfu/ml represent mean ± standard deviation for three independent determinations. Standard deviations are shown by the error bars. (b) Morphology of mock-infected or RSV-infected cells at the indicated post-infection time periods. (c) MTT cell viability assay of cells infected with RSV for 36 h. MTT assay values are mean ± standard deviation of 6 wells and triplicate experiments. Uninfected (-) cells indicate 100% cell viability. (d) RSV mediated apoptosis at 24 h post-infection. % Apoptosis represents the % of cells that are undergoing apoptosis (i.e. positive for annexin V staining). The values represent mean ± SD for three determinations.