Migration of HNF1α-inhibited HepG2 cells. A: Boyden chamber assay. HepG2 cells transfected with control or HNF1α siRNA for 72 h were seeded in insert upper chamber and left to migrate towards medium with serum for 16 h. B: Wound-healing assay. Confluent HepG2 cells transfected with control or HNF1α siRNA for 48 h, were scratched with pipette tips and pictures were taken every day for 3 days. Representative images from three independent experiments are shown. Scale bar 100 μm.